Publications

2018

Deficiency of the T cell regulator CBL-B aggravates atherosclerosis by inducing CD8+ T cell-mediated macrophage death
Seijkens, T.T.P., Poels, K., Meiler, S., van Tiel, C.M., Kusters, P.J.H., Reiche, M., Atzler, D., Winkels, H.,  Tjwa, M., Poelman, H., Slütter, B., Kuiper, J., Gijbels, M., Kuivenhoven, J.A., Matic, L.P., Paulsson-Berne, G., Hedin, U., Hansson, G.K., Nicolaes, G.A.F., Daemen, M.J.A.P., Weber, C., Gerdes, N., de Winther, M., and Esther Lutgens (2018) Eur Heart J, accepted for publication

Inhibiting  inflammation  with  myeloid  cell-specific  nanobiologics  promotes  organ transplant  acceptance
Braza, M.S.,  van  Leent, M.M.T., Lameijer, M., Sanchez- Gaytan, B.L.,  Arts, R.J.W., Pérez-Medina, C., Conde, P., Garcia, M.R., Gonzalez-Perez, M., Brahmachary, M., Fay, F., Kluza, E., Kossatz, S., Dress, R.J.,  Salem, F., Rialdi, A., Reiner, T., Boros, P., Strijkers, G.J., Calcagno, C.C., Ginhoux, F., Marazzi, I., Lutgens, E., Nicolaes, G.A.F., Weber, C., Swirski, F.K., Nahrendorf, M., Fisher, E.A., Duivenvoorden, R., Fayad, Z.A., Netea, M.G., Mulder, W.J.M., Ochando, J (2018) Immunity, accepted for publication

Child-onset thrombotic thrombocytopenic purpura caused by p.R498C and p.G259PfsX133 mutations
Schelpe A., Orlando C., Ercig B., Geeroms C., Pareyn I., Vandeputte N., Velásquez P., Leydi C.,  Roose E., Fostier K., Nicolaes G., Deckmyn H., De Meyer S., Vanhoorelbeke K., Jochmans K. (2018) European Journal of Haematology, May 15. doi: 10.1111/ejh.13094

Chrono-pharmacological targeting of the CCL2-CCR2 axis ameliorates atherosclerosis
Winter, C., Silvestre-Roig, C., Ortega-Gomez, A., Lemnitzer, P., Poelmann, H., Schumski,A., Winter, J., Drechsler, M., de Jong, R., Immler, R., Sperandio, M., Hristov, M., Zeller, T., Nicolaes, G.A.F., Weber, C., Viola, J.R., Hidalgo, A., Scheiermann, C., Soehnlein, O. (2018) Cell Metabolism, Jul 3;28(1):175-182.e5. doi: 10.1016/j.cmet.2018.05.002

TRAF-STOPping atherosclerosis: targeting of CD40-induced TRAF6 signaling in macrophages reduces (established) atherosclerosis
Seijkens, T.T.P., van Tiel, C.M., Kusters, P.J.H., Pascal, J.H., Atzler, D., Soehnlein, O., Zarzycka, B., Aarts, S.A.B.M., Lameijer, M., M.A., Gijbels, M.J., Beckers, L., den Toom, M., Slütter, B., Kuiper, J., Duchene, J., Aslani, M., Megens, R., van ‘t Veer, C., Kooij, G., Schrijver, R., Hoekseman, M., Boon, L., Fay, F., Tang, J., Baxter, S., Jongejan, A., Moerland, P.D., Vriend, G., Bleijlevens, B., Fisher, E.A., Duivenvoorden, R., Gerdes, N., de Winther, M.P.J., Nicolaes, G.A.F., Mulder, W.J.M., Weber, C., Lutgens, E.
(2018) The Journal of the American College of Cardiology, 71(5) 527-42

Insights into 3D-structure of ADAMTS13: a stepping stone towards novel therapeutic treatment of TTP
Ercig, B, Wichapong, K, Reutelingsperger, CPM, Vanhoorelbeke, K, Voorberg, J, Nicolaes, GAF
(2017) Journal of Thrombosis and Haemostasis, Jan;118(1):28-41.
PMID:29304523

2017

Inhibition of CD40-TRAF6 interactions by the small molecule inhibitor 6877002 reduces neuroinflammation.
Aarts SABM, Seijkens TTP, Kusters PJH, van der Pol SMA, Zarzycka B, Heijnen PDAM, Beckers L, den Toom M, Gijbels MJJ, Boon L, Weber C, de Vries HE, Nicolaes GAF, Dijkstra CD, Kooij G, Lutgens E.
(2017) J Neuroinflammation. 2017 May 12;14(1):105.
PMID:28494768

Chemokine interactome mapping enables tailored intervention in acute and chronic inflammation.
von Hundelshausen P, Agten SM, Eckardt V, Blanchet X, Schmitt MM, Ippel H, Neideck C, Bidzhekov K, Leberzammer J, Wichapong K, Faussner A, Drechsler M, Grommes J, van Geffen JP, Li H, Ortega-Gomez A, Megens RT, Naumann R, Dijkgraaf I, Nicolaes GAF, Döring Y, Soehnlein O, Lutgens E, Heemskerk JW, Koenen RR, Mayo KH, Hackeng TM, Weber C.
(2017) Sci Transl Med. 2017 Apr 5;9(384).
PMID:28381538

Presence of Cytotoxic Extracellular Histones in Machine Perfusate of Donation After Circulatory Death Kidneys.
van Smaalen, T.C., Beurskens, D., Hoogland, E.R.P., Winkens, B.,  Christiaans, M.H.L, Reutelingsperger, C.P., van Heurn, L.W.E., and G.A.F. Nicolaes
(2017) Transplantation, Apr;101(4):e93-e101.
PMID:27906828

2016

Structure-based design of peptidic inhibitors of the interaction between CCL5 and HNP1.
Wichapong, K., Alard, J., Ortega-Gomez, A., Weber, C., Hackeng, T.M., Soehnlein, O. and G.A.F. Nicolaes.
(2016) J Med Chem, May 12;59(9):4289-301.PMID:26871718

Pharmacological targeting of AMP-activated protein kinase and opportunities for computer-aided drug design.
Miglianico, M., Nicolaes, G.A.F., Neumann, D.
(2016) J Med Chem, 2016 Apr 14;59(7):2879-93. PMID:26510622

Stabilisation of protein-protein interaction complexes through small molecules.
Zarzycka, B., Kuenemann, M.A., Miteva, M., Nicolaes, G.A.F., Vriend, G., Sperandio, O.
(2016) Jan;21(1):48-57. Drug Disc Today. PMID:26434617

iPPI-DB: an online database of modulators of protein-protein interactions.
Labbé, C.M., Kuenemann, M.A., Zarzycka, B., Vriend, G., Nicolaes, G.A.F., Lagorce, D., Miteva, M.A., Villoutreix, B.O., Sperandio, O.
(2016) Nucleic Acids Res.; Jan 4;44(D1):D542-7. PMID:26432833

2015

Recruitment of classical monocytes can be inhibited by disturbing heteromers of neutrophil HNP1 and platelet CCL5.
Alard, J.-E., Ortega-Gomez, A., Wichapong, K., Bongiovanni, D., Horckmans, M., Megens, R.T.A., Leoni, G., Ferraro, B., Rossaint, J., Paulin, N., Ng, J., Ippel, H., Suylen, D., Hinkel, R., Blanchet, X., Gaillard, F., D’Amico. M., von Hundelshausen, P., Zarbock, A., Scheiermann, C., Hackeng, T.M., Steffens, S., Kupatt, C., Weber, C., Nicolaes, G.A.F., and O. Soehnlein
(2015) Sience Transl Med.; 7(317):317ra196. PMID:26659570

Extracellular histone H3 levels are inversely correlated with antithrombin levels and platelet counts and are associated with mortality in sepsis patients.
Wildhagen, K.C.A.A., Wiewel, M.A., Schultz, M.J., Horn, J., Schrijver, R., Reutelingsperger, C.P.M., van der Poll, T., Nicolaes, G.A.F.
(2015) Thromb. Res.; 136(3):542-7. PMID:26232351

Identification of small molecule inhibitors against SecA by structure-based virtual ligand screening.
De Waelheyns, E., Segers, K., Sardis, M., Anné, J., Nicolaes, G.A.F. and Economou, A.
(2015), Journal of Antibiotics Nov;68(11):666-73. PMID:25990955

Targeting the adaptive immune system: new strategies in the treatment of atherosclerosis.
Zarzycka, B., Nicolaes, G.A.F., Lutgens, E.
(2015) Expert Review of Clinical Pharmacology, 8(3):297-313. PMID:25843158

Two Novel Mutations in the SLC25A4 Gene in a Patient with Mitochondrial Myopathy.
Körver-Keularts IM, de Visser M, Bakker HD, Wanders RJ, Vansenne F, Scholte HR, Dorland L, Nicolaes GAF, Spaapen LM, Smeets HJ, Hendrickx AT, van den Bosch BJ.
(2015) JIMD Rep. 22; 39-45. PMID:25732997

Discovery of small molecule CD40-TRAF6 inhibitors.
Zarzycka, B., Seijkens, T., Nabuurs, S.B., Ritschel, T., Grommes, J., Soehnlein, O., Schrijver, R., van Tiel, C.M., Hackeng, T.M., Weber, C., Giehler, F., Kieser, A., Lutgens, E., Vriend, G. and Nicolaes, G.A.F.
(2015), Journal of Chemical Information and Modeling, Feb 23;55(2):294-307. PMID:25622654

Molecular simulation studies of human coagulation factor VIII C domain-mediated membrane binding.
Du, J., Wichapong, K., Hackeng, T. and Nicolaes, G.A.F.
(2015) Thrombosis and Haemostasis, Jan 27;113(2):373-84. PMID:25354705

2014

The Structure Function of the Death Domain of human IRAK-M.
Du, J., Nicolaes, G.A.F., Kruijswijk, D., Versloot, M., van der Poll, T. and van ’t Veer, C.
(2014), Cell Communication and Signaling.
Dec 7;12(1):77. PMID:25481771

Blocking CD40-Traf6 interactions by small molecule inhibitor 6860766 ameliorates the complications of diet induced obesity in mice.
Van den Berg, S., Seijkens, T., Kusters, P., Zarzycka, B., Beckers, L., den Toom, M., Gijbels, M., Chatzigeorgiou, A., Weber, C., de Winther, M., Chavakis, T., Nicolaes, G.A.F. and Lutgens, E.
(2014), Int J Obes (Lond). 39(5):782-90. PMID:25394307

Understanding the functional difference between growth arrest-specific protein 6 (GAS6) and protein S (PROS1): an evolutionary approach.
Studer, R.A., Opperdoes, F.R., Nicolaes, G.A.F., Mulder, A.B. and Mulder, R.
(2014) Open Biol. Oct;4(10). pii: 140121. doi: 10.1098/rsob.140121. PMID:25339693

Factor Va alternative conformation reconstruction using Atomic Force Microscopy.
Chaves, R.C., Dahmane, S., Odorico, M., Nicolaes, G.A.F., and J.-L. Pellequer
(2014) Thromb Haemost. 2014 Sep 4;112(6). PMID:25185589

Effects of exogenous recombinant APC in mouse models of ischemia reperfusion injury and of atherosclerosis.
Wildhagen, K.C.A.A., Schrijver, R., Beckers, L., ten Cate, H., Reutelingsperger, C.P., Lutgens, E., Nicolaes, G.A.F.
(2014) PLOS ONE, Jul 17;9(7):e101446. PMID:25032959

Identification of novel small molecule inhibitors of activated protein C.
Sperandio, O.,Wildhagen, K.C.A.A., Schrijver, R., Wielders, S., Villoutreix, B.O., Nicolaes, G.A.F.
(2014) Thromb. Res.; 133(6):1105-14. PMID:24513148

Blocking CD40‐TRAF6 signaling is a novel therapeutic target in obesity‐associated insulin resistance.
Chatzigeorgiou, A., Seijkens, T., Zarzycka, B., Engel, D., Poggi, M., van den Berg, S., van den Berg, S., Soehnlein, O., Winkels, H., Beckers, L., Lievens, D., Driessen, A., Kusters, P., Biessen, E., Garcia-Martin, R., Klotzsche-von Ameln, A., Gijbels, M., Noelle, R., Boon, L., Hackeng, T.M., Schulte, K., Xu, A., Vriend, G., Nabuurs, S., Chung, K., Willems van Dijk, K., Rensen, P.C.N., Gerdes, N., Winther, M., Block, N.L., Schally, A.V., Weber, C., Bornstein, S.R., Nicolaes, G.A.F., Chavakis, T., Lutgens, E. shared last authors
(2014) Proc. Natl. Acad. Sci., U S A. 2014 Feb 18;111(7):2686-91. PMID:24492375

Non-anticoagulant heparin prevents histone-mediated cytotoxicity in vitro and improves survival in sepsis.
Wildhagen, K.C.A.A., García de Frutos, P., Reutelingsperger, C.P., Schrijver, R., Aresté, C., Ortega-Gómez, A., Deckers, N.M., Hemker, H.C., Soehnlein, O., Nicolaes, G.A.F.
(2014) Blood 13;123(7):1098-101. PMID:24264231

Rational design of small molecules targeting the C2 domain of coagulation factor VIII.
Nicolaes, G.A.F., Kulharia, M., Voorberg, J., Kaijen, P.H., Wroblewska, A., Wielders, S., Schrijver, R., Sperandio, O., Villoutreix, B.O.
(2014) Blood; 123(1):113-20. PMID:24227818

Optimization of compound ranking for structure-based virtual ligand screening using an established FRED-Surflex consensus approach.
Du, J., Bleylevens, I.W.M., Bitorina, A.V., Wichapong, K., Nicolaes, G.A.F.
(2014) Chemical Biology and Drug Design; 83(1):37-51. PMID:23941463

2013

Factor Xa-induced activation of factor V is of paramount importance in initiating the coagulation system: lessons from a tick salivary protein.
Schuijt, T.J., Bakhtiari, K., Daffre, S., DePonte, K., Wielders, S.J.H., Marquart, J.A., Hovius, J.W., van der Poll, T., Fikrig, E., Bunce, M.W., Camire, R.M., Nicolaes, G.A.F., Meijers, J.C.M. and van ’t Veer, C.
(2013) Circulation;128(3):254-66. PMID:23817575

Two novel mutations in the prothrombin gene identified in a patient with compound heterozygous type 1/2 prothrombin deficiency.
Kuijper P.H., Schellings M.W., van de Kerkhof D., Nicolaes G.A.F., Reitsma P., Halbertsma F., Dors N.
(2013) Haemophilia, May28. PMID:23711336

2012

Comparative Analysis of Pharmacophore Screening Tools.
Sanders MP, Barbosa AJ, Zarzycka B, Nicolaes GA, Klomp JP, de Vlieg J, Del Rio A.
(2012) J Chem Inf Model. 52(6): 1607-20 PMID: 22646988

Goodpasture Antigen-binding Protein/ceramide Transporter Binds to Human Serum Amyloid P-component and is presentin Brain Amyloid Plaques.
Mencarelli C, Bode GH, Losen M, Kulharia M, Molenaar PC, Veerhuis R, Steinbusch HW, De Baets MH, Nicolaes GAF, Martinez-Martinez P.
(2012) J Biol Chem 287(18): 14897-911 PMID: 22396542    

De structuur-functie relatie van geactiveerd protein C: wat mutaties ons kunnen leren.
Wildhagen, K.C.A.A., Lutgens, E.,  Loubele S.T.B.G., ten Cate, H.,Nicolaes, G.A.F.
(2012) Tromnibus, 40;2:29-32 (in Dutch)   

2011

APC Resistance. Gerry A.F. Nicolaes (2011). in: Thrombophilia, Andrea Luigi Tranquilli (Ed.), ISBN: 978-953-307-872-4, InTech
http://www.intechopen.com/articles/show/title/apc-resistance

The structure-function relationship of activated protein C: lessons from natural and engineered mutations.
Wildhagen K.C.A.A., Lutgens E., Loubele S.T.G.B, ten Cate H and Nicolaes G.A.F.
(2011) Thromb. Haemostas, Nov 10;106(6) PMID: 22072231

Engineered annexin A5 variants with impaired cell entry for molecular imaging of apoptosis using pretargeting strategies.
Ungethum L., Kenis H., Nicolaes G.A.F., Autin L., Stoilova-McPhie S., Reutelingsperger C.P.
(2011) J Biol Chem. 286(3):1903-1910 PMID: 21078669

Riboflavin-responsive oxidative phosphorylation complex I deficiency caused by defective ACAD9: new function for an old gene.
Gerards M., van den Bosch B.J., Danhauser K., Serre V., van Weeghel M., Wanders R.J., Nicolaes G.A.F., Sluiter W., Schoonderwoerd K., Scholte H.R., Prokisch H., Rötig A., de Coo I.F., Smeets H.J.
(2011) Brain. 134(1): 210-219 PMID: 20929961

2010

Trp2313-His2315 of the FVIII C2 domain are involved in membrane binding: Structural analysis of a complex between the C2 domain and an inhibitor of membrane binding.
Liu, Z., Lin, L., Yuan, C., Nicolaes, G.A.F., Chen, L., Meehan, E.J., Furie, B., Furie, B., Huang, M.
(2010) J. Biol. Chem.285(12):8824-9 PMID: 20089867

Inhibition of thrombin formation by active site Ser360 to Ala-substituted activated protein C.
Nicolaes, G.A.F., Bock, P.E., Segers, K., Wildhagen, K.C.A.A., Dahlback, B., Rosing, J.
(2010) J. Biol. Chem. May 18. 48. PMID: 20484050

The D2194G mutation is responsible for increased levels of FV1 in carriers of the factor V R2 haplotype.
Yamazaki, T. Okada, H., Balling, K.W., Dahlbäck, B., Nicolaes, G.A.F.
(2010) Thromb. Haemostas. Oct;104(4):860-1

2009

FVIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of FVIII.
Heinz, S., Schüttrumpf, J., Simpson, J.C., Pepperkok, R., Nicolaes, G.A.F., Seifried, E.,  Tonn, T.
(2009) Thrombosis and Haemostasis, Thromb Haemost. (2009);102(5):925-35 PMID: 19888531

Factor IX Mutants with Enhanced Catalytic Activity.
Hartmann, R.,  Dockal, M., Kammlander, W., Fiedler, C., Panholzer, E., Nicolaes, G.A.F., Rosing, J. and Scheiflinger, F.
(2009) Journal of Thromb. Haemost., 7(10):1656-62 PMID: 19656280

2008

Multimerin 1 inhibits thrombin generation and binds factor V and Va with high affinity.
Jeimy, S.B., Fuller, N., Quin-Allen, M.A., Tasneem, S. Segers, K., Stafford, A.R., Weitz, J.I., Camire, R.M., Nicolaes, G.A.F., Kane, W.H., Hayward, C.P.M.
(2008) J Biol Chem, Thromb Haemost., 100(6):1058-67.PMID: 19132231

Screening Outside the Catalytic Site: Inhibition of Macromolecular Inter-actions Through Structure-Based Virtual Ligand Screening Experiments.
Sperandio O, Miteva MA, Segers K, Nicolaes GA, Villoutreix BO.
(2008) Open Biochem J. 2008;2:29-37. PMID: 18949072

Identification of surface epitopes of human coagulation factor Va which are important for interaction with activated protein C and heparin.
Segers, K., Dahlbäck, B., Rosing, J., Nicolaes, G.A.F.
(2008) J Biol Chem, 283(33):22573-81 PMID: 18519572

Re-evaluation of the role of the protein S-C4b binding protein complex in activated protein C-catalyzed factor Va-inactivation.
Maurissen, L.F.A., Thomassen, M.C.L.G.D., Nicolaes, G.A.F., Dahlbäck, B., Tans, G., Rosing, J. and  Hackeng, T.M.
(2008) Blood 111(6):3034-41. PMID: 18160668

PROS1 Analysis in 87 Pedigrees with Hereditary Protein S Deficiency Demonstrates Striking Genotype-Phenotype Associations.
ten Kate, M., Platteel, M., Mulder, R., Terpstra, P. Nicolaes, G.A.F., Reitsma, P., van der Steege, G., van der Meer, J.
(2008) Human Mutation, 29(7), 939-47. PMID: 18435454

Defining the structure of membrane-bound human blood coagulation Factor Va.
Stoilova-McPhie, S., Awosile. F., Parmenter, C.D.J., Segers, K., Villoutreix, B.O., Nicolaes, G.A.F.
(2008) J Thromb Haemost. 6: 76-82. PMID: 17949476

2007

The role of thrombin exosites I and II in the activation of human coagulation factor V.
Segers, K., Dahlbäck, B., Bock, P.E., Tans, G.,Rosing, J., Nicolaes, G.A.F.
(2007) J Biol Chem.  282(47):33915-33924. PMID: 17878169

Coagulation factor V and thrombophilia: background and mechanisms.
Segers, K., Dahlbäck, B., Nicolaes, G.A.F.
(2007) Thrombosis and Haemostasis, 98(3): 530-542. PMID: 17849041

Design of protein-membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V.
Segers, K., Sperandio, O., Sack, M., Fischer, R., Miteva, M., Rosing, J., Nicolaes, G.A.F., Villoutreix, B.O.
(2007) Proc. Natl. Acad. Sci. 104 (31):12697-702. PMID: 17646652

Expression of allosteric linkage between the sodium ion binding site and exosite I of thrombin during prothrombin activation.
Kroh, H.K., Tans, G., Nicolaes, G.A.F., Rosing, J., Bock, P.E.
(2007) JBC, J Biol Chem. 282(22):16095-104. PMID: 17430903

2006

Biochemical, molecular characterization and glyco-proteomic analyses of alpha1-proteinase inhibitor products used for replacement therapy.
Kolarich, D., Turecek, P.L., Weber, A., Mitterer, A., Graninger, M., Matthiessen, P., Nicolaes, G.A.F., Altmann, F., Schwarz, H.P.
(2006) Transfusion 46(11):1959-77.  PMID: 17076852

A structural model of the SHBG domain of human variant protein S Heerlen.
Nicolaes, G.A.F., Hackeng, T.M., Segers, K., Rosing, J.
(2006) Thromb Haemost. 96(4):538-40. PMID: 17003935

Structural Models of the Snake Venom Factor V activators from Daboia russelli and Daboia lebetina.
Segers, K., Rosing, J., Nicolaes, G.A.F.
(2006). Proteins 64(4):968-84. PMID: 16807918

Activation of Factor V by Venom Proteases.
Nicolaes,G.A.F., Rosing, J.
(2006) Toxin Reviews 25(3):217-234. DOI: 10.1080/15569540600567289

2004

Identification of the MMRN1 binding region within the C2 domain of human factor V.
Jeimy, S.B., Woram, R.A., Fuller, N., Quinn-Allen, M.A., Nicolaes, G.A.F., Dahlbäck, B., Kane, W.H., Hayward, C.P.
(2004) J Biol Chem., 279(49):51466-71. PMID: 15452129

Altered inactivation pathway of factor Va by activated protein C in the presence of heparin.
Nicolaes G.A.F., Sörensen, K.W., Friedrich, U., Tans, G., Rosing, J.,  Autin L. , Dahlbäck B., Villoutreix B.O.
(2004) Eur J Biochem., 271(13):2724-36. PMID: 15206937

Impaired APC-cofactor activity of factor V is a major component of the APC resistance associated with the FV Leiden (R506Q) and R2 (H1299R) mutations.
Castoldi, E., Brugge, J.M., Nicolaes, G.A.F., Tans, G., Rosing, J.
(2004) Blood, 103(11):4173-9. PMID: 14976057

Functional Properties of Recombinant Factor V mutated in a Potential Calcium-Binding Site.
Sörensen, K.W., Nicolaes, G.A.F., Villoutreix, B.O., Yamazaki, T., Tans, G., Rosing, J., Dahlbäck, B.
(2004) Biochemistry, 43(19):5803-10. PMID: 15134454

Welke factor laat ons bloed stollen?
Nicolaes, G.A.F.
(2004) Chemisch2Magazine, 100(2): 18-19 (in Dutch)

Theoretical and experimental study of the Asp2194Gly mutation in the C2 domain of coagulation factor V.
Miteva, M.A., Brugge, J.M., Rosing, J., Nicolaes, G.A.F., Villoutreix, B.O.
(2004) Biophys. J., 86(1): 488-98. PMID: 14695293

2003

Congenital and Acquired Activated Protein C Resistance.
Nicolaes, G.A.F., Dahlbäck, B.
(2003). Seminars in Vascular Medicine, 3, 33-46. PMID: 15199491

Activated protein C resistance (FVLeiden) and thrombosis: factor V mutations causing hypercoagulable states.
Nicolaes, G.A.F., Dahlbäck, B.
(2003) Hematology and Oncology Clinics of North America, 17, 1-25. PMID: 12627662

2002

Structure-function studies of blood coagulation proteins using recombinant human factor V molecules.
Nicolaes, G.A.F., Tans, G., Rosing, J. Progress Report CARIM, University Maastricht (2002) 50-54.

Molecular basis of quantitative factor V deficiency associated with factor V R2 haplotype.
Yamazaki, T., Nicolaes, G.A.F., Winther Sørensen, K., Dahlbäck, B.
(2002) Blood, 100, 2515-2521. PMID: 12239164

Reduced factor V concentration and altered factor V1/factor V2 ratio do not fully explain R2-associated APC-resistance.
Govers-Riemslag, J., Castoldi, E., Nicolaes, G.A.F., Tans, G., Rosing, J.
(2002) Thrombosis and Haemostasis, 88, 444-449. PMID: 12353074

Factor V and Thrombotic disease: description of a Janus-faced protein.
Nicolaes, G.A.F., Dahlbäck, B.
(2002) Arteriosclerosis, Thrombosis, and Vascular Biology, 22(4), 530-538. PMID: 11950687

2001

Factor V.
Nicolaes G.A.F., Dahlbäck B.
(2001) Encyclopedia of Molecular Medicine, T.E. Creighton, ed., John Wiley and Sons, Inc., New York, 1231-1234.  ISBN 10: 0471374946

Introduction to structural bioinformatics; applications to blood coagulation factor V and factor VIII.
Bruno O. Villoutreix, Gerry A. F. Nicolaes, Martine Aiach
(2001) Hématologie, 7(4), 287-296. (in French) link

Secondary substrate-binding exosite in the serine protease domain of activated protein C important for cleavage at Arg-506 but not at Arg-306 in factor Va.
Friedrich U., Nicolaes G.A.F., Villoutreix B.O., Dahlbäck B.
(2001) Journal of Biological Chemistry, 276(25), 23105-23108. PMID: 11309403

2000

The second Laminin G-type domain of protein S is indispensable for full cofactor activity in activated protein C-catalyzed inactivation of factor Va and factor VIIIa.
Evenäs, P., Garcia de Frutos, P., Nicolaes, G.A.F., Dahlbäck, B.
(2000) Thrombosis and Haemostasis, 84(2),271-277. PMID: 10959700

Mutations in a potential phospholipid binding loop in the C2 domain of factor V affecting the assembly of the prothrombinase complex.
Nicolaes, G.A.F., Villoutreix, B.O., Dahlbäck, B.
(2000) Blood Coagulation & Fibrinolysis, 11, 89-100. PMID: 10691103

1999

Acquired APC resistance and oral contraceptives: A randomised cross-over study of two low dose oral contraceptives.
Rosing, J., Middeldorp, S., Curvers, J., Thomassen, M.C.L.G.D., Nicolaes, G.A.F., Meijers, J., Bouma, B.N., Büller, H.R., Prins, M.H., Tans, G.
(1999) the Lancet, 354, 2036-2040. PMID: 10636369

Partial glycosylation of Asn2181 in human factor V as cause of molecular and functional heterogeneity.
Nicolaes, G.A.F., Villoutreix, B.O., Dahlbäck, B.
(1999) Biochemistry, 38(41), 13584-13591. PMID: 10521265

Acquired APC resistance and oral contraceptives: Differences between two functional tests.
Curvers, J., Thomassen, M.C.L.G.D., Nicolaes, G.A.F., van Oerle, R., Hamulyak, K., Hemker, H.C., Tans, G., Rosing, J.
(1999) British Journal of Haematology, 105(1), 88-94. PMID: 10233368

1997

Regulation of thrombin formation by activated protein C: effect of the FVLeiden mutation.
Tans, G., Nicolaes, G.A.F., Rosing, J.
(1997) Seminars in Hematology, 34(3), 244-255. PMID: 9241709

Human factor Va1 and Va2: Properties in the procoagulant- and anticoagulant pathways.
Hoekema, L, Nicolaes, G.A.F., Tans, G., Hemker, H.C., Rosing, J.
(1997) Biochemistry, 36(11), 3331-3335. PMID: 9116011

Oral Contraceptives and venous thrombosis: different sensitivities to activated protein C in women using second and third generation oral contraceptives.
Rosing, J., Tans, G., Nicolaes, G.A.F., Thomassen M.C.L.G.D., Van Oerle, R., Van der Ploeg, P.M.E.N., Heijnen, P., Hamulyak, K., Hemker, H.C.
(1997) British Journal of Haematology 97, 233-238. PMID: 9136971

Effect of activated protein C on thrombin generation and on the thrombin potential in the plasma of normal and APC resistant individuals.
Nicolaes, G.A.F., Thomassen, M.C.L.G.D., Tans, G., Rosing, J., Hemker, H.C.
(1997) Blood Coagulation & Fibrinolysis 8, 28-38. PMID: 9105635

1996

A prothrombinase-based assay for detection of resistance to activated protein C.
Nicolaes, G.A.F., Thomassen, M.C.L.G.D., van Oerle, R., Hamulyak, K., Hemker, H.C., Tans, G., Rosing, J.
(1996) Thrombosis and Haemostasis 76(3), 404-410. PMID: 8883278

1995

Effects of protein S and factor Xa on peptide bond cleavages during inactivation of factor Va and factor VaR506Q by activated protein C.
Rosing, J., Hoekema, L., Nicolaes, G.A.F., Thomassen, M.C.L.G.D., Hemker, H.C., Varadi, K., Schwarz, H.P., Tans, G.
(1995) Journal of Biological Chemistry 270, 27852-27858. PMID: 7499257

Peptide bond cleavages and loss of functional activity during inactivation of factor Va and factor VaR506Q by activated protein C.
Nicolaes, G.A.F., Tans, G., Thomassen, M.C.L.G.D., Hemker, H.C., Pabinger, I., Varadi, K., Schwarz, H.P., Rosing, J.
(1995) Journal of Biological Chemistry 270, 21158-21166.  PMID: 7673148

1994

Activation of human factor V by meizothrombin.
Tans,G., Nicolaes, G.A.F., Thomassen, M.C.L.G.D., Hemker, H.C., van Zonneveld, A-J., Pannekoek, H., Rosing, J.
(1994) Journal of Biological Chemistry 269, 15969-15972. PMID: 8206891

Acylation of porcine pancreatic PLA2 influences penetration and substrate head- group binding, depending on the position of the acylated lysine in the enzyme molecule.
Lugtigheid, R.B., Nicolaes, G.A.F., Veldhuizen, E.J.A., Slotboom, A.J., Verheij, H.M., de Haas, G.H.
(1993) European Journal of Biochemistry 216(2), 519-525. PMID: 8375390